Comparative Analysis of bla_KPC Expression in Tn4401 Transposons and the Tn3-Tn4401 Chimera.

ABSTRACT: Two basic structures that carry the bla_KPC gene, the Tn4401 transposon and the Tn3-Tn4401 chimera, have been identified within and outside China. However, the different bla_KPC expression levels and promoter activities of these two structures are not completely understood. We constructed Tn4401a, Tn4401b, and Tn3-Tn4401 chimera recombinants and found that the imipenem (IPM) and meropenem (MEM) MICs for the Escherichia coli transformants carrying the chimera were 2-fold higher than for those carrying Tn4401b but 2-fold lower than for those carrying Tn4401a In addition to the promoter P1, we characterized a novel potential promoter sequence (PX) in the chimera using 5' rapid amplification

SUBMITTER: Huang J 

PROVIDER: S-EPMC6496108 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature