Inhibition of cell proliferation, migration and invasion by a glioma-targeted fusion protein combining the p53 C terminus and MDM2-binding domain.

ABSTRACT:

Objectives

The aim of this study was to develop multifunctional fusion proteins for targeting and delivering therapy elements into glioma cells.

Materials and methods

Multifunctional fusion proteins were expressed in Escherichia coli and purified using Ni-NTA resin affinity chromatography. Human glioma cells and primary astrocytes were used to analyse their functions. Targeting proteins location to glioma cells was observed by confocal microscopy. Effects of cell viability and proliferation were evaluated using the Cell Counting Kit 8 and colony formation assays. Glioma cell migration and invasion were assessed using transwell assays, and apoptosis was analysed by flow cytometry. In addition, changes in expression of proteins related to the cell cycle and apoptosis were dete

SUBMITTER: Yu J 

PROVIDER: S-EPMC6496415 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature