Dataset Information
MicroRNA-762 promotes breast cancer cell proliferation and invasion by targeting IRF7 expression.
Ontology highlight
ABSTRACT: Objectives
miRNAs play crucial roles in human tumourigenesis. This study was performed to measure expression and function of miR-762 in breast cancer.Materials and methods
Expression of miR-762 in breast tissues and cell lines (SK-BR-3, DA-MB-435s, MCF-7 and MDA-MB-231, HBL-100) was measured by using real-time RT-PCR. We restored expression of miR-762 in MCF-7 cells to measure its functional roles. Luciferase assays were performed to reveal the target gene of miR-762.Results
Expression of miR-762 was high in both breast cancer cell lines and specimens, and its overexpression increased breast cancer cell proliferation and invasion. Interferon regulatory factor 7 (IRF7) is a direct target of miR-762 and overexpression of miR-762 reduced expression of IRF7. Moreover, IRF7 was repressed, its levels inversely correlated to miR-762 expression. IRF7 rescued miR-762-induced cell invasion and proliferation.Conclusions
These results demonstrate that miR-762 tumour effect was achieved by targeting IRF7 in human breast cancer specimens.
SUBMITTER: Li Y
PROVIDER: S-EPMC6496530 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Publications
microRNA-762 promotes breast cancer cell proliferation and invasion by targeting IRF7 expression.
Cell proliferation 20151102 6
<h4>Objectives</h4>miRNAs play crucial roles in human tumourigenesis. This study was performed to measure expression and function of miR-762 in breast cancer.<h4>Materials and methods</h4>Expression of miR-762 in breast tissues and cell lines (SK-BR-3, DA-MB-435s, MCF-7 and MDA-MB-231, HBL-100) was measured by using real-time RT-PCR. We restored expression of miR-762 in MCF-7 cells to measure its functional roles. Luciferase assays were performed to reveal the target gene of miR-762.<h4>Results< ...[more]