Genistein and daidzein repress adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells via Wnt/β-catenin signalling or lipolysis.

ABSTRACT:

Objectives

One aspect of the effects of isoflavones against fat deposition might be at least associated with the mechanism by which Wnt/β-catenin signalling inhibits adipocyte differentiation. However, it remains completely unknown as to whether isoflavones might influence Wnt signalling during commitment of pluripotent mesenchymal stem cells (MSCs) to adipose lineages. In the present study, we have investigated the mechanisms underlying effects of genistein and daidzein, the major soy isoflavones, on anti-adipogenic Wnt/β-catenin signalling.

Materials and methods

Adipose tissue-derived (AD) MSCs were exposed continuously to genistein and daidzein (0.01-100 μm) during adipogenic differentiation (21 days). An oestrogen antagonist, ICI 182,780, was used to determine whether or

SUBMITTER: Kim MH 

PROVIDER: S-EPMC6496531 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature