Ex vivo expansion of cord blood progenitors impairs their short-term and long-term repopulating activity associated with transcriptional dysregulation of signalling networks.

ABSTRACT:

Objectives

Cord blood (CB) has been established to be an alternative source of haematopoietic stem/progenitor cells (HPC) for transplantation. The number of HPC per CB unit is limited, which results in engraftment delay. Ex vivo expansion of HPC improvement must overcome this.

Materials and methods

Flow cytometry was used to extensively phenotype HPC pre- and post-expansion and CFDA-SE staining was used to track cell divisions. The NSG mouse model was employed in transplantation studies to determine long and short term repopulation in human cells. Gene array analysis was used to evaluate signalling pathways regulated following ex vivo expansion of HPC.

Results

expansion of CD34(+) HPC impaired their regenerative function. In this xenograft transplantation model we sho

SUBMITTER: Holmes T 

PROVIDER: S-EPMC6496543 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature