Differential proteome and phosphoproteome signatures in human T-lymphoblast cells induced by sirolimus.

ABSTRACT:

Objectives

The present study was designed to investigate early proteome and phosphoproteome changes during inhibition of lymphocyte proliferation induced by sirolimus (SRL).

Materials and methods

Proliferation assays were conducted using human CCRF-CEM T lymphoblasts under different SRL concentrations. Total protein lysates after SRL treatment were used to identify significantly regulated proteins and phosphorylated proteins by 2-DE and Q-TOF Ultima Global mass spectrometer.

Results and conclusions

Incubation with 2.5 micromol/l SRL resulted in a approximately 70% inhibition of cell proliferation. Cells incubated with 2.5 micromol/l for 30 min showed a differential phosphorylation pattern with one higher (TCPQ) and six lower phosphorylation signals (TBA1B, VIME, HNRPD

SUBMITTER: Schultze FC 

PROVIDER: S-EPMC6496580 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature