Global gene expression reveals a set of new genes involved in the modification of cells during erythroid differentiation.

ABSTRACT:

Objectives

Erythroid differentiation is a dynamic process in which a pluripotent stem cell undergoes a series of developmental changes that commit it to a specific lineage. These alterations involve changes in gene expression profiles. In this study, gene expression profiles during differentiation of human erythroid cells of a normal blood donor were evaluated using SAGE.

Materials and methods

Global gene expression was evaluated in cells collected immediately before addition of erythropoietin (0 h) and 192 and 336 h after addition of this hormone. Real-time PCR was used to evaluate activation of differentially expressed genes.

Results

The data indicate that global aspects of the transcriptome were similar during differentiation of the majority of the genes and that a

SUBMITTER: da Cunha AF 

PROVIDER: S-EPMC6496675 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature