Antibody Fab-Fc properties outperform titer in predictive models of SIV vaccine-induced protection.

ABSTRACT: Characterizing the antigen-binding and innate immune-recruiting properties of the humoral response offers the chance to obtain deeper insights into mechanisms of protection than revealed by measuring only overall antibody titer. Here, a high-throughput, multiplexed Fab-Fc Array was employed to profile rhesus macaques vaccinated with a gp120-CD4 fusion protein in combination with different genetically encoded adjuvants, and subsequently subjected to multiple heterologous simian immunodeficiency virus (SIV) challenges. Systems analyses modeling protection and adjuvant differences using Fab-Fc Array measurements revealed a set of correlates yielding strong and robust predictive performance, while models based on measurements of response magnitude alone exhibited significantly inferior perform

SUBMITTER: Pittala S 

PROVIDER: S-EPMC6497031 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature