Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution.

ABSTRACT: Optogenetic techniques use light-responsive proteins to study dynamic processes in living cells and organisms. These techniques typically rely on repurposed naturally occurring light-sensitive proteins to control subcellular localization and activity. We previously engineered two optogenetic systems, the light activated nuclear shuttle (LANS) and the light-inducible nuclear exporter (LINX), by embedding nuclear import or export sequence motifs into the C-terminal helix of the light-responsive LOV2 domain of Avena sativa phototropin 1, thus enabling light-dependent trafficking of a target protein into and out of the nucleus. While LANS and LINX are effective tools, we posited that mutations within the LOV2 hinge-loop, which connects the core PAS domain and the C-terminal helix, would furthe

SUBMITTER: Lerner AM 

PROVIDER: S-EPMC6497050 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature