Characterization and analysis of a novel diguanylate cyclase PA0847 from Pseudomonas aeruginosa PAO1.

ABSTRACT: Background: As a central signaling molecule, cyclic diguanylate (c-di-GMP) is found to regulate various bacterial phenotypes, especially those involved in pathogen infection and drug resistance. Noticeably, many microbes have up to dozens of proteins that are involved in c-di-GMP metabolism. This apparent redundancy and the relevant functional specificity have become the focus of research. While a number of these proteins have been identified and investigated, the functions of PA0847, a PAS and GGDEF domain-containing protein from Pseudomonas aeruginosa PAO1, remain unclear. Materials and methods: In the current study, microbiology, biochemistry and structural biology methods were applied to characterize the gene/protein of PA0847. Results: We showed that PA0847 affects bacterial motility

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6497469 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature