SIRT2, ERK and Nrf2 Mediate NAD⁺ Treatment-Induced Increase in the Antioxidant Capacity of PC12 Cells Under Basal Conditions.

ABSTRACT: NAD⁺ (oxidized form of nicotinamide adenine dinucleotide) administration is highly beneficial in numerous models of diseases and aging. It is becoming increasingly important to determine if NAD⁺ treatment may directly increase the antioxidant capacity of cells under basal conditions. In the current study, we tested our hypothesis that NAD⁺ can directly enhance the antioxidant capacity of cells under basal conditions by using PC12 cells as a cellular model. We found that NAD⁺ treatment can increase the GSH/GSSG ratios in the cells under basal conditions. NAD⁺ can also increase both the mRNA and protein level of γ-glutamylcysteine ligase (γ-GCL)-a key enzyme for glutathione synthesis, which appears to be mediated by the NAD⁺-i

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6497790 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature