Clustered Kv2.1 decreases dopamine transporter activity and internalization.

ABSTRACT: The dopamine transporter (DAT) regulates dopamine neurotransmission via reuptake of dopamine released into the extracellular space. Interactions with partner proteins alter DAT function and thereby dynamically shape dopaminergic tone important for normal brain function. However, the extent and nature of these interactions are incompletely understood. Here, we describe a novel physical and functional interaction between DAT and the voltage-gated K⁺ channel Kv2.1 (potassium voltage-gated channel subfamily B member 1 or KCNB1). To examine the functional consequences of this interaction, we employed a combination of immunohistochemistry, immunofluorescence live-cell microscopy, co-immunoprecipitation, and electrophysiological approaches. Consistent with previous reports, we f

SUBMITTER: Lebowitz JJ 

PROVIDER: S-EPMC6497934 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature