Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing.

ABSTRACT:

Objective

MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive.

Methods

RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment.

Results

The results showed total o

SUBMITTER: Liu Z 

PROVIDER: S-EPMC6498074 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature