Genome-Wide Identification of Fitness Factors in Seawater for Edwardsiella piscicida.

ABSTRACT: Marine pathogens are transmitted from one host to another through seawater. Therefore, it is important for marine pathogens to maintain survival or growth in seawater. However, little is known about how marine pathogens adapt to living in seawater environments. Here, transposon insertion sequencing was performed to explore the genetic determinants of Edwardsiella piscicida survival in seawater at 16 and 28°C. Seventy-one mutants with mutations mainly in metabolism-, transportation-, and type III secretion system (T3SS)-related genes showed significantly increased or impaired fitness in 16°C water. In 28°C seawater, 63 genes associated with transcription and translation, as well as energy production and conversion, were essential for optimal survival of the bacterium. In particular,

SUBMITTER: Wei L 

PROVIDER: S-EPMC6498171 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature