¹³C Labeling of Nematode Worms to Improve Metabolome Coverage by Heteronuclear Nuclear Magnetic Resonance Experiments.

ABSTRACT: Nuclear magnetic resonance (NMR) spectroscopy is widely used as a metabolomics tool, and 1D spectroscopy is overwhelmingly the commonest approach. The use of 2D spectroscopy could offer significant advantages in terms of increased spectral dispersion of peaks, but has a number of disadvantages-in particular, heteronuclear 2D spectroscopy is often much less sensitive than 1D NMR. One factor contributing to this low sensitivity in ¹³C/¹H heteronuclear NMR is the low natural abundance of the ¹³C stable isotope; as a consequence, where it is possible to label biological material with ¹³C, there is a potential enhancement of sensitivity of up to around 90fold. However, there are some problems that can reduce the advantages otherwise gained-in particul

SUBMITTER: Geier FM 

PROVIDER: S-EPMC6498324 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature