Enhanced production of styrene by engineered Escherichia coli and in situ product recovery (ISPR) with an organic solvent.

ABSTRACT:

Background

Styrene is a large-volume commodity petrochemical, which has been used in a wide range of polymer industry as the main building block for the construction of various functional polymers. Despite many efforts to produce styrene in microbial hosts, the production titers are still low and are not enough to meet the commercial production of styrene.

Results

Previously, we developed a high L-phenylalanine producer (E. coli YHP05), and it was used as a main host for de novo synthesis of styrene. First, we introduced the co-expression system of phenylalanine-ammonia lyase (PAL) and ferulic acid decarboxylase (FDC) genes for the synthesis of styrene from L-phenylalanine. Then, to minimize cell toxicity and enhance the recovery of styrene, in situ product recovery (ISPR) w

SUBMITTER: Lee K 

PROVIDER: S-EPMC6498506 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature