Establishment of a sensitive TaqMan-based real-time PCR assay for porcine circovirus type 3 and its application in retrospective quarantine of imported boars to China.

ABSTRACT: Porcine circovirus type 3 (PCV3) is a novel pathogen first identified in the United States in 2016. As there is a high possibility that no clinical signs of infection are observed in the host, an accurate and sensitive method is needed for quarantine on numerous live pigs especially for international pig trade. In this study, a TaqMan-based real-time PCR assay specifically for PCV3 was established without cross-reactions with other non-targeted pig viruses. The sensitivity of the current approach is about 1.5 × 10¹  copies μL^-1 plasmid DNA while the sensitivity of the conventional PCR is about 1.5 × 10²  copies μL^-1 plasmid DNA. Further, this assay was applied in the retrospective quarantine on serum samples of 601 commercial live boars imported

SUBMITTER: Feng C 

PROVIDER: S-EPMC6498530 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature