A substitution in the pre-S1 promoter region is associated with the viral regulation of hepatitis B virus.

ABSTRACT:

Background

Much evidence has demonstrated the influence of Hepatitis B virus (HBV) mutations on the clinical course of HBV infection. As large (L) protein plays a crucial role for viral entry, we hypothesized that mutations in the pre-S1 promoter region might affect the expression of L protein and subsequently change the biological characters of virus.

Methods

Patients infected with genotype C HBV were enrolled for analysis. HBV DNA sequences were inserted into a TA cloning vector and analyzed. To evaluate the effects of mutations in the pre-S1 promoter region, promoter activity and the expression of mRNA and L protein were analyzed using HepG2 cells.

Results

In total, 35 patients were enrolled and 13 patients (37.1%) had a single base substitution in the pre-S1 promo

SUBMITTER: Ogura S 

PROVIDER: S-EPMC6498540 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature