Unknown

Dataset Information

0

Tristetraprolin specifically regulates the expression and alternative splicing of immune response genes in HeLa cells.


ABSTRACT: BACKGROUND:Tristetraprolin (TTP) is an RNA binding protein that plays a critical role in regulating proinflammatory immune responses by destabilizing target mRNAs via binding to their AU-rich elements (AREs) in the 3'-UTRs of mRNAs. A recent CLIP-seq study revealed that TTP-binding sites are enriched in the intronic regions of RNA. TTP is also a nuclear protein that exhibits putative DNA-binding activity. These features suggested that TTP might regulate gene transcription and/or alternative splicing of pre-mRNAs in the absence of stimulation. RESULTS:To elucidate the regulatory pattern of TTP, we cloned and overexpressed the human TTP-encoding gene, ZFP36, in HeLa cells in the absence of inflammatory stimuli. The transcriptomes of the control and ZFP36-overexpressing cells were sequenced and subjected to analysis and validation. Upon ZFP36 overexpression, the expression of genes associated with innate immunity, including those in the type I interferon signaling pathway and viral response, were specifically upregulated, implying a transcriptional regulatory mechanism associated with the predicted DNA binding activity of TTP. TTP preferentially regulated the alternative splicing of genes involved in the positive regulation of the I-?B/NF-?B cascade and the TRIF-dependent toll-like receptor, MAPK, TNF, and T cell receptor signaling pathways. CONCLUSIONS:Our findings indicated that TTP may regulate the immune response via the regulation of alternative splicing and potentially transcription, which greatly expands the current understanding of the mechanisms of TTP-mediated gene regulation.

SUBMITTER: Tu Y 

PROVIDER: S-EPMC6498542 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Tristetraprolin specifically regulates the expression and alternative splicing of immune response genes in HeLa cells.

Tu Yafang Y   Wu Xiongfei X   Yu Fengyun F   Dang Jianzhong J   Wang Juan J   Wei Yaxun Y   Cai Zhitao Z   Zhou Zhipeng Z   Liao Wenliang W   Li Lian L   Zhang Yi Y  

BMC immunology 20190502 1


<h4>Background</h4>Tristetraprolin (TTP) is an RNA binding protein that plays a critical role in regulating proinflammatory immune responses by destabilizing target mRNAs via binding to their AU-rich elements (AREs) in the 3'-UTRs of mRNAs. A recent CLIP-seq study revealed that TTP-binding sites are enriched in the intronic regions of RNA. TTP is also a nuclear protein that exhibits putative DNA-binding activity. These features suggested that TTP might regulate gene transcription and/or alternat  ...[more]

Similar Datasets

2019-03-24 | GSE114216 | GEO
2020-03-12 | GSE130053 | GEO
| PRJNA470575 | ENA
| PRJNA533642 | ENA
| S-EPMC7859996 | biostudies-literature
| S-EPMC7551351 | biostudies-literature
| S-EPMC6537309 | biostudies-literature
| S-EPMC6549092 | biostudies-literature
| S-EPMC7551455 | biostudies-literature
2023-01-11 | GSE157125 | GEO