Identification and differential expression of piRNAs in the gonads of Amur sturgeon (Acipenser schrenckii).

ABSTRACT:

Objective

Sturgeons are considered living fossils, and have a very high conservation and economic value. Studies on the molecular mechanism of sturgeon gonadal development and sex differentiation would not only aid in understanding vertebrate sex determination but also benefit sturgeon aquaculture. Piwi-interacting RNAs (piRNAs) have been shown to function in germline or gonadal development. In this study, we performed small RNA deep sequencing and microarray hybridization to identify potential sturgeon piRNAs.

Methods

Male and female sturgeon gonads were collected and used for small RNA sequencing on an Illumina HiSeq platform with the validation of piRNA expression by microarray chip. The program Bowtie and k-mer scheme were performed to filter small RNA reads and d

SUBMITTER: Yuan L 

PROVIDER: S-EPMC6499119 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature