Overexpression of the ChVDE gene, encoding a violaxanthin de-epoxidase, improves tolerance to drought and salt stress in transgenic Arabidopsis.

ABSTRACT: To investigate the protective mechanism of violaxanthin de-epoxidase (VDE) zeaxanthin in Cerasus humilis under drought and salt-stress conditions, we cloned the entire cDNA sequence of ChVDE from C. humilis and generated ChVDE-overexpression (OE) and ChVDE-complementation (CE) Arabidopsis plants. The open reading frame of ChVDE contained 1,446 bp nucleotides and encoded 481 amino acids. The ChVDE showed the highest similarity with those of Camellia sinensis and Citrus sinensis. Subcellular localization analysis showed that ChVDE was located in the chloroplasts. OE plants showed stronger root growth and higher levels of total chlorophyll as compared to WT and VDE mutant (npq1-2) plants. Moreover, the relative de-ep

SUBMITTER: Sun LN 

PROVIDER: S-EPMC6499860 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature