Identification of a novel thrombospondin-related anonymous protein (BoTRAP2) from Babesia orientalis.

ABSTRACT:

Background

The thrombospondin-related anonymous protein (TRAP) was first discovered in the sporozoite of Plasmodium falciparum and TRAP family proteins are secreted by micronemes and transported to the parasite surface to participate in the invasion process. Various TRAP proteins have been identified in apicomplexan protozoans, but there have been few reports about TRAP proteins in Babesia orientalis.

Methods

The functional domain of TRAP2 in B. orientalis was cloned, sequenced, characterized and compared to the TRAP sequences of related apicomplexan parasites. The functional domain of BoTRAP2 was truncated, named BoTRAP2-1, and then cloned into the pET-28a expression vector. Rabbit anti-rBoTRAP2-1 polyclonal antibody was produced by immunizing three rabbits. Western blot an

SUBMITTER: Zhan X 

PROVIDER: S-EPMC6500065 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature