Size matters: three methods for estimating nuclear size in mycorrhizal roots of Medicago truncatula by image analysis.

ABSTRACT:

Background

The intracellular accommodation of arbuscular mycorrhizal (AM) fungi involves a profound molecular reprogramming of the host cell architecture and metabolism, based on the activation of a symbiotic signaling pathway. In analogy with other plant biotrophs, AM fungi are reported to trigger cell cycle reactivation in their host tissues, possibly in support of the enhanced metabolic demand required for the symbiosis.

Results

We here compare the efficiency of three Fiji/ImageJ image analysis plugins in localizing and quantifying the increase in nuclear size - a hallmark of recursive events of endoreduplication - in M. truncatula roots colonized by the AM fungus Gigaspora margarita. All three approaches proved to be versatile and upgradeable, allowing the investigation

SUBMITTER: Carotenuto G 

PROVIDER: S-EPMC6500585 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature