Knockdown of KLF5 promotes cisplatin-induced cell apoptosis via regulating DNA damage checkpoint proteins in non-small cell lung cancer.

ABSTRACT: BACKGROUND:Previous research has revealed that Krüppel-like factor 5 (KLF5) may affect DNA damage repair pathways; however, the associated molecular mechanisms are unclear. METHODS:The expression of KLF5 was studied by immunohistochemical staining in paired tumour and normal tissues from 90 patients with ESCC. We studied the effects of KLF5 knockdown on cell proliferation and apoptosis with or without cisplatin treatment in A549 and H1299 cell lines. Moreover, we examined the effect of KLF5 on the DNA damage response. RESULTS:KLF5 was significantly overexpressed in non-small cell lung cancer (NSCLC) tissues, and high KLF5 expression predicted poor prognosis for NSCLC patients. The inhibition of KLF5 markedly augmented cisplatin-induced cell apoptosis. In addition, we observed that KLF5 kno

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6501027 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature