Characterization of genomic DNA of lactic acid bacteria for activation of plasmacytoid dendritic cells.

ABSTRACT:

Background

Lactococcus lactis strain Plasma (LC-Plasma) possesses strong stimulatory activity for plasmacytoid dendritic cells (pDCs) via the TLR9-Myd88 pathway. To reveal the effective lactic acid bacteria (LAB) genome structure for pDCs stimulatory activity, we performed in vitro screening, using randomly selected 200 bp DNA fragments from the LC-Plasma genome.

Results

We found that the CpG motif copy number in the fragments was positively and significantly correlated with pDCs stimulatory activity (R = 0.491, p < 0.01). However, the determination coefficient (R²) was 0.24, which means other factors affecte activity. We found that the G + C contents of the fragment showed a significant negative correlation with activity (R = - 0.474, p < 0.01). The correlation b

SUBMITTER: Horie A 

PROVIDER: S-EPMC6501324 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature