Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding.

ABSTRACT: During cell-to-cell communications, the interplay between physical and biochemical cues is essential for informational exchange and functional coordination, especially in multicellular organisms. However, it remains a challenge to visualize intercellular signaling dynamics in single live cells. Here, we report a photonic approach, based on laser microscissors and Förster resonance energy transfer (FRET) microscopy, to study intercellular signaling transmission. First, using our high-throughput screening platform, we developed a highly sensitive FRET-based biosensor (SCAGE) for Src kinase, a key regulator of intercellular interactions and signaling cascades. Notably, SCAGE showed a more than 40-fold sensitivity enhancement than the original biosensor in live mammalian cells. Next, upon loca

SUBMITTER: Wang P 

PROVIDER: S-EPMC6502247 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature