Characterization of single microvesicles in plasma from glioblastoma patients.

ABSTRACT:

Background

Extracellular vesicles (EV) are shed by tumor cells but little is known about their individual molecular phenotypes and heterogeneity. While exosomes have received considerable attention, much less is known about larger microvesicles. Here we profile single microvesicles (MV) and exosomes from glioblastoma (GB) cells and MV from the plasma of patients.

Methods

EV secreted from mouse glioma GL261 and human primary GBM8 cell lines as well as from the plasma of 8 patients with diagnoses of GB and 2 healthy controls were isolated and processed for single vesicle analysis. EV were immobilized on glass slides and the heterogeneity of vesicle and tumor markers were analyzed at the single vesicle level.

Results

We show that (i) MV are abundant, (ii) only a minority

SUBMITTER: Fraser K 

PROVIDER: S-EPMC6502493 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature