Aspergillus niger citrate exporter revealed by comparison of two alternative citrate producing conditions.

ABSTRACT: Currently, there is no consensus regarding the mechanism underlying Aspergillus niger citrate biosynthesis and secretion. We hypothesise that depending on the experimental setup, extracellular citrate accumulation can have fundamentally different underlying transcriptomic landscapes. We show that varying the amount and type of supplement of an arginine auxotrophic A. niger strain results in transcriptional down-regulation of citrate metabolising enzymes in the condition in which more citrate is accumulated extracellularly. This contrasts with the transcriptional adaptations when increased citrate production is triggered by iron limitation. By combining gene expression data obtained from these two very distinct experimental setups with hidden Markov models and transporter homology approache

SUBMITTER: Odoni DI 

PROVIDER: S-EPMC6502548 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature