Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis.

ABSTRACT:

Objective

The study was designed to determine the cytotoxic effect of gallic acid (GA), obtained by the hydrolysis of tannins, on mice TM4 Sertoli cells apoptosis.

Methods

In the present study, non-tumorigenic mice TM4 Sertoli cells were treated with different concentrations of GA for 24 h. After treatment, cell viability was evaluated using WST-1, mitochondrial dysfunction, cells apoptosis and necrosis was detected using JC-1, Hoechst 33342 and propidium iodide staining. The expression levels of Cyclin B1, proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (BAX), and Caspase-3 were also detected by quantitative real-time polymerase chain reaction and Western-blotting.

Results

The results showed that 20 to 400 μM GA inhibited viability of TM4 Sertol

SUBMITTER: Li W 

PROVIDER: S-EPMC6502723 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature