MTHFR C677T polymorphism analysis: A simple, effective restriction enzyme-based method improving previous protocols.

ABSTRACT:

Background

5,10-Methylentetrahydrofolate reductase (MTHFR) C677T polymorphism is one of the most studied genetic variations in the human genome. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is one of the most used techniques to characterize the point mutations in genomic sequences because of its suitability and low cost. The most widely used method for the MTHFR C677T polymorphism characterization was developed by Frosst et al. (1995) but appears to have some technical limitations. The aim of this study was to propose a novel PCR-RFLP method for the detection of this polymorphism.

Methods

In order to retrieve all published articles possibly describing any PCR-RFLP methods useful to analyze MTHFR C677T polymorphism, we performed systematic que

SUBMITTER: Antonaros F 

PROVIDER: S-EPMC6503068 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature