Long-wavelength native-SAD phasing: opportunities and challenges.

ABSTRACT: Native single-wavelength anomalous dispersion (SAD) is an attractive experimental phasing technique as it exploits weak anomalous signals from intrinsic light scatterers (Z < 20). The anomalous signal of sulfur in particular, is enhanced at long wavelengths, however the absorption of diffracted X-rays owing to the crystal, the sample support and air affects the recorded intensities. Thereby, the optimal measurable anomalous signals primarily depend on the counterplay of the absorption and the anomalous scattering factor at a given X-ray wavelength. Here, the benefit of using a wavelength of 2.7 over 1.9 Å is demonstrated for native-SAD phasing on a 266 kDa multiprotein-ligand tubulin complex (T₂R-TTL) and is applied in the structure determination of an 86 kDa helicase Sen

SUBMITTER: Basu S 

PROVIDER: S-EPMC6503925 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature