Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing.

ABSTRACT: In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene expression to maintenance of genomic integrity. However, the molecular mechanisms by which actin operates in the nucleus remain poorly understood. Here, we have used two complementary mass spectrometry (MS) techniques, AP-MS and BioID, to identify binding partners for nuclear actin. Common high-confidence interactions highlight the role of actin in chromatin-remodeling complexes and identify the histone-modifying complex human Ada-Two-A-containing (hATAC) as a novel actin-containing nuclear complex. Actin binds directly to the hATAC subunit KAT14, and modulates its histone acetyl transferase activity in vitro and i

SUBMITTER: Viita T 

PROVIDER: S-EPMC6503952 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature