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Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin reveals that extracellular Ca²⁺, Mg²⁺ and production of cAMP are involved in toxicity.

ABSTRACT: Bacillus thuringiensis (Bt) produces pore forming toxins that have been used for pest control in agriculture for many years. However, their molecular and cellular mode of action is still unclear. While a first model - referred to as the pore forming model - is the most widely accepted scenario, a second model proposed that toxins could trigger an Mg²⁺-dependent intracellular signalling pathway leading to cell death. Although Cry1Ca has been shown to form ionic pores in the plasma membrane leading to cell swelling and death, we investigated the existence of other cellular or molecular events involved in Cry1Ca toxicity. The Sf9 insect cell line, derived from Spodoptera frugiperda, is highly and specifically sensitive to Cry1Ca. Through a selection program we develop

SUBMITTER: Castella C

PROVIDER: S-EPMC6503997 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Dataset Information

Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin reveals that extracellular Ca²⁺, Mg²⁺ and production of cAMP are involved in toxicity.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin reveals that extracellular Ca2+, Mg2+ and production of cAMP are involved in toxicity.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin reveals that extracellular Ca²⁺, Mg²⁺ and production of cAMP are involved in toxicity.