Dominant-negative inhibition of canonical Notch signaling in trophoblast cells does not disrupt placenta formation.

ABSTRACT: Proper development and function of the mammalian placenta requires interactions between embryo-derived trophoblasts and uterine endothelial cells to form mosaic vessels that facilitate blood flow to a developing conceptus. Notch signaling utilizes a cell-cell contact dependent mechanism to drive cell behaviors, such as differentiation and invasion. In mice, Notch2 is needed for proper placentation and embryo survival. We used transgenic mice with a dominant-negative form of Mastermind-like1 and Cyp19-Cre and Tpbpa-Cre drivers to inhibit canonical Notch signaling in trophoblasts. Both Cre drivers resulted in robust placental expression of dominant-negative Mastermind-like1. All pregnancies progressed beyond mid-gestation and morphological analyses of placentas revealed no differences betwee

SUBMITTER: Shawber CJ 

PROVIDER: S-EPMC6504009 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature