Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season.

ABSTRACT:

Study objective

To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission.

Methods

This single-center prospective study recruited adult patients attending the emergency department for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran rapid RT-PCR assays using a dedicated device (cobas Liat, Roche Diagnostics, Meylan, France) located at triage. The same swab sample was also analyzed in the department of virology using conventional RT-PCR techniques. Patients were included 24 hours-a-day, 7 days-a-week. The primary outcome was the diagnostic accuracy of the rapid RT-PCR assay performed at triage.

Results

A total of 187 patients were included over 11 days in January 2018. Median age

SUBMITTER: Maignan M 

PROVIDER: S-EPMC6504036 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature