Experimental steering of electron microscopy studies using prior X-ray computed tomography.

ABSTRACT: Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) can provide unrivalled high-resolution images of specific features and volumes of interest. However, the regions interrogated are typically very small, and sample preparation is both time-consuming and destructive. Here we consider how prior X-ray micro-computed tomography (microCT) presents an opportunity to increase the efficiency of electron microscopy in biology. We demonstrate how it can be used to; select the most promising samples and target site-specific locations; provide a wider context of the location being interrogated (multiscale correlative imaging); guide sample preparation and 3D imaging schemes; as well as quantify the effects of destructive sample preparation and staining procedures. We present

SUBMITTER: Starborg T 

PROVIDER: S-EPMC6504073 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature