Digital Image Analysis of BAP-1 Accurately Predicts Uveal Melanoma Metastasis.

ABSTRACT:

Purpose

Reduced nuclear expression of BRCA1 associated protein 1 (BAP-1) is associated with a high risk for metastasis in uveal melanoma. Manual assessment of the expression level may face issues with interobserver reproducibility. This could be improved with digital image analysis (DIA).

Methods

Thirty enucleated eyes with uveal melanoma from the Emory Eye Center (Atlanta, GA; years 2009-2017) were included and stained with BAP-1. Retrospective data on patient and tumor characteristics were retrieved. Patients were randomized to a training or validation cohort. Their tumor sections were digitally scanned and scored for percentage of BAP-1-positive cells with the QuPath Bioimage analysis software.

Results

Interobserver concordance was 75% (Cohen's κ 0.52) with manual

SUBMITTER: Stalhammar G 

PROVIDER: S-EPMC6504204 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature