Development of a new monoclonal antibody specific to mouse Vγ6 chain.

ABSTRACT: There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene-transfected T-cell line lacking TCR as well as of Vγ1^- Vγ4^- Vγ5^- Vγ7^- γδ T cells and the CD3^high TCRδ^int γδ T cells in various organs. The location of Vγ6⁺ γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but amelio

SUBMITTER: Hatano S 

PROVIDER: S-EPMC6504751 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature