Semiquantitative Nucleic Acid Test with Simultaneous Isotachophoretic Extraction and Amplification.

ABSTRACT: Nucleic acid amplification tests (NAATs) provide high diagnostic accuracy for infectious diseases and quantitative results for monitoring viral infections. The majority of NAATs require complex equipment, cold chain dependent reagents, and skilled technicians to perform the tests. This largely confines NAATs to centralized laboratories and can significantly delay appropriate patient care. Low-cost, point-of-care (POC) NAATs are especially needed in low-resource settings to provide patients with diagnosis and treatment planning in a single visit to improve patient care. In this work, we present a rapid POC NAAT with integrated sample preparation and amplification using electrokinetics and paper substrates. We use simultaneous isotachophoresis (ITP) and recombinase polymerase amplification (

SUBMITTER: Bender AT 

PROVIDER: S-EPMC6504979 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature