Quantitative proteomic analyses of CD4⁺ and CD8⁺ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls.

ABSTRACT:

Background

Multiple sclerosis (MS) is an autoimmune, neuroinflammatory disease, with an unclear etiology. However, T cells play a central role in the pathogenesis by crossing the blood-brain-barrier, leading to inflammation of the central nervous system and demyelination of the protective sheath surrounding the nerve fibers. MS has a complex inheritance pattern, and several studies indicate that gene interactions with environmental factors contribute to disease onset.

Methods

In the current study, we evaluated T cell dysregulation at the protein level using electrospray liquid chromatography-tandem mass spectrometry to get novel insights into immune-cell processes in MS. We have analyzed the proteomic profiles of CD4⁺ and CD8⁺ T cells purified from whol

SUBMITTER: Berge T 

PROVIDER: S-EPMC6505067 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature