Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum.

ABSTRACT: Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reported, we observed that for growth, the addition of vitamins to media already containing yeast extract was required, an indication that these are fastidious microorganisms. Elimination of complex components and individual vitamins from the medium revealed that the only organic compounds required for growth were pantothenate, biotin and thiamine. Analysis of the genome sequences revealed that three genes were missing from pantothenate and thiamine biosynthetic pathways, and five genes were absent from the pathway for biotin biosynthesis. Prototrophy

SUBMITTER: Annan FJ 

PROVIDER: S-EPMC6505512 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature