Targeted Quantitative Proteomic Approach for Probing Altered Protein Expression of Small GTPases Associated with Colorectal Cancer Metastasis.

ABSTRACT: Genes encoding the small GTPases of the Ras superfamily are among the most frequently mutated or dysregulated in human cancer. No systematic studies, however, have yet been conducted for assessing the implications of small GTPases in the metastatic transformation of colorectal cancer (CRC). By utilizing a recently established high-throughput multiple-reaction monitoring (MRM)-based workflow together with stable isotope labeling by amino acids in cell culture (SILAC), we investigated comprehensively the relative expression of the small GTPase proteome in a pair of matched primary/metastatic CRC cell lines (SW480/SW620). Among the 83 quantified small GTPases, 25 exhibited at least a 1.5-fold difference in protein expression in SW480 and SW620 cells. In particular, SAR1B protein was found to

SUBMITTER: Huang M 

PROVIDER: S-EPMC6506370 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature