Illumina and Nanopore methods for whole genome sequencing of hepatitis B virus (HBV).

ABSTRACT: Advancing interventions to tackle the huge global burden of hepatitis B virus (HBV) infection depends on improved insights into virus epidemiology, transmission, within-host diversity, drug resistance and pathogenesis, all of which can be advanced through the large-scale generation of full-length virus genome data. Here we describe advances to a protocol that exploits the circular HBV genome structure, using isothermal rolling-circle amplification to enrich HBV DNA, generating concatemeric amplicons containing multiple successive copies of the same genome. We show that this product is suitable for Nanopore sequencing as single reads, as well as for generating short-read Illumina sequences. Nanopore reads can be used to implement a straightforward method for error correction that reduces th

SUBMITTER: McNaughton AL 

PROVIDER: S-EPMC6506499 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature