Detection of helminth ova genera using in-situ biosynthesis of gold nanoparticles.

ABSTRACT: In this study, a presumptive colorimetric method was used to detect and differentiate the ova of two major soil transmitted helminths in wastewater, Ascaris and Trichuris. Gold nanoparticles were synthesised following the reduction of tetrachloroauric acid by the surface moiety of Ascaris suum, resulting in a colour change. In contrast there was no colour change with Trichuris suis indicating the absence of gold nanoparticle synthesis. Analysis of the ova using scanning electron microscopy (SEM) revealed that the synthesis of nanoparticles on the surface of ova was confirmed as gold nanoparticles (91 w/w %) by energy dispersive X-ray analysis (EDX). This study indicated that the surface moieties of helminth ova could be a potential target for ova detection and d

SUBMITTER: Ravindran VB 

PROVIDER: S-EPMC6506560 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature