VANGL2 regulates luminal epithelial organization and cell turnover in the mammary gland.

ABSTRACT: The VANGL family of planar cell polarity proteins is implicated in breast cancer however its function in mammary gland biology is unknown. Here, we utilized a panel of Vang1 and Vangl2 mouse alleles to examine the requirement of VANGL family members in the murine mammary gland. We show that Vang1CKO^Δ/Δ glands display normal branching while Vangl2^flox/flox and Vangl2^Lp/Lp tissue exhibit several phenotypes. In MMTV-Cre;Vangl2^flox/flox glands, cell turnover is reduced and lumens are narrowed. A Vangl2 missense mutation in the Vangl2^Lp/Lp tissue leads to mammary anlage sprouting defects and deficient outgrowth with transplantation of anlage or secondary tissue fragments. In successful Vangl2^Lp/Lp outgrowths, three morphological

SUBMITTER: Smith P 

PROVIDER: S-EPMC6506599 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature