Mitochondrial genome and transcriptome analysis of five alloplasmic male-sterile lines in Brassica juncea.

ABSTRACT:

Background

Alloplasmic lines, in which the nuclear genome is combined with wild cytoplasm, are often characterized by cytoplasmic male sterility (CMS), regardless of whether it was derived from sexual or somatic hybridization with wild relatives. In this study, we sequenced and analyzed the mitochondrial genomes of five such alloplasmic lines in Brassica juncea.

Results

The assembled and annotated mitochondrial genomes of the five alloplasmic lines were found to have virtually identical gene contents. They preserved most of the ancestral mitochondrial segments, and the same candidate male sterility gene (orf108) was found harbored in mitotype-specific sequences. We also detected promiscuous sequences of chloroplast origin that were conserved among plants of the Brassicaceae,

SUBMITTER: Wu Z 

PROVIDER: S-EPMC6507029 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature