MicroRNA profiling of plasma exosomes from patients with ovarian cancer using high-throughput sequencing.

ABSTRACT: To the best of our knowledge, the microRNA (miR/miRNA) expression profile of plasma exosomes in ovarian cancer has not been previously studied. The aim of the present study was to investigate the practicality of using plasma exosomal miRNAs as novel serological biomarkers of ovarian cancer. In the study, exosome-like vesicles were purified from the plasma of patients with ovarian cancer and healthy women using differential centrifugation. The purified vesicles, ranging from 50-100 nm in size, were identified as exosomes by transmission electron microscopy and western blotting. High-throughput sequencing demonstrated that 65 known miRNAs, 34 of which were upregulated and 31 downregulated, were differentially expressed between patients with ovarian cancer and healthy women (P<0.05; fold chan

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6507395 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature