Altered nucleosome positions in maize haplotypes and mutants of a subset of SWI/SNF-like proteins.

ABSTRACT: Chromatin remodelers alter DNA-histone interactions in eukaryotic organisms and have been well characterized in yeast and Arabidopsis. While there are maize proteins with similar domains as known remodelers, the ability of the maize proteins to alter nucleosome position has not been reported. Mutant alleles of several maize proteins (RMR1, CHR101, CHR106, CHR127, and CHR156) with similar functional domains to known chromatin remodelers were identified. Altered gene expression of Chr101, Chr106, Chr127, and Chr156 was demonstrated in plants homozygous for the mutant alleles. These mutant genotypes were subjected to nucleosome position analysis to determine whether misregulation of putative maize chromatin proteins would lead to altered DNA-histone interactions. N

SUBMITTER: Stroud LK 

PROVIDER: S-EPMC6508530 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature