Effects of overexpression of jasmonic acid biosynthesis genes on nicotine accumulation in tobacco.

ABSTRACT: Nicotine is naturally synthesized in tobacco roots and accumulates in leaves as a defense compound against herbivory attack. Nicotine biosynthesis pathway has been extensively studied with major genes and enzymes being isolated and functionally characterized. However, the molecular regulation of nicotine synthesis has not been fully understood. The phytohormone jasmonic acid (JA) mediates many aspects of plant defense responses including nicotine biosynthesis. In this study, five key genes (AtLOX2, AtAOS, AtAOC2, AtOPR3, AtJAR1) involved in JA biosynthesis from Arabidopsis were individually overexpressed, and a JA-Ile hydrolysis-related gene, NtJIH1, was suppressed by RNAi approach, to understand their effects on nicotine accumulation in tob

SUBMITTER: Chen H 

PROVIDER: S-EPMC6508566 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature