PKM2 enhances cancer invasion via ETS-1-dependent induction of matrix metalloproteinase in oral squamous cell carcinoma cells.

ABSTRACT:

Objectives

This study aimed at investigating the molecular mechanism underlying PKM2-mediated cancer invasion.

Materials & methods

To optimize the investigation of PKM2-specific effects, we used two immortalized oral cell lines. The two cell lines drastically differed in PKM2 expression level, particularly in the level of nuclear PKM2, and subsequently in glucose metabolism and tumorigenicity.

Results

Knockdown of PKM2 reduced not only the glucose metabolism but also the invasive activity by curtailing the expressions of matrix metalloproteinases (MMP): PKM2 could modulate MMP-9 expression by regulating ETS-1 inside the nucleus. These results were further confirmed in an oral squamous cell carcinoma (OSCC) cell line. In correspondence with in vitro findings, clinicopa

SUBMITTER: Park YJ 

PROVIDER: S-EPMC6508653 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature